human colon epithelial cell line ccd841 con  (ATCC)

Journal: International Journal of Molecular Sciences

Article Title: Chemical Characterization and In Vitro Antioxidant, Anti-Inflammatory, and Colon Cancer-Preventive Potential of a Polysaccharide Fraction from Macrolepiota procera

doi: 10.3390/ijms26209978

Figure Lengend Snippet: Cytotoxic influence of crude polysaccharides from M. procera on both normal and cancer colon cell lines. Human colon epithelial CCD841 CoN cells and human colon adenocarcinoma Caco-2, LS180, and HT-29 cells were treated with the crude polysaccharides Mp-CPS at concentrations of 10, 25, 50, and 100 μg/mL for 24 h. Mp-CPS cytotoxicity was examined photometrically by the LDH assay. Results are presented as mean ± SEM of at least 4 measurements. ** p < 0.01 versus control, *** p < 0.001 versus control, one-way ANOVA test; post-test: Dunnett.

Article Snippet: The human colon epithelial cell line CCD841 CoN and the human colon adenocarcinoma cell line Caco-2 were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA).

Techniques: Lactate Dehydrogenase Assay, Control

Journal: International Journal of Molecular Sciences

Article Title: Chemical Characterization and In Vitro Antioxidant, Anti-Inflammatory, and Colon Cancer-Preventive Potential of a Polysaccharide Fraction from Macrolepiota procera

doi: 10.3390/ijms26209978

Figure Lengend Snippet: Antiproliferative effect of crude polysaccharides from M. procera on both normal and cancer colon cell lines. Human colon epithelial CCD841 CoN cells and human colon adenocarcinoma Caco-2, LS180, and HT-29 cells were treated with the crude polysaccharides Mp-CPS at concentrations of 10, 25, 50, and 100 μg/mL for 96 h. Mp-CPS’s impact on cell metabolic activity was measured photometrically by the MTT assay. Results are presented as mean ± SEM of at least 4 measurements. * p < 0.05 versus control, ** p < 0.01 versus control, *** p < 0.001 versus control, one-way ANOVA test; post-test: Dunnett.

Article Snippet: The human colon epithelial cell line CCD841 CoN and the human colon adenocarcinoma cell line Caco-2 were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA).

Techniques: Activity Assay, MTT Assay, Control

Journal: International Journal of Molecular Sciences

Article Title: Phytochemical Profiling of Extracts from Rare Potentilla Species and Evaluation of Their Anticancer Potential

doi: 10.3390/ijms24054836

Figure Lengend Snippet: Influence of obtained extracts on the viability of CCD841 CoN and LS180 cell lines. The cells were exposed for 48 h to the culture medium alone (control). extract at concentrations of 25–250 µg/mL. or 25 μM 5-fluorouracil (5-FU; positive control). Metabolic activity of investigated cell lines in response to tested compounds was examined photometrically by MTT assay. Results are presented as mean ± SEM of at least 5 measurements. * p < 0.05; ** p < 0.01; *** p < 0.001 vs. control. # p < 0.05; ## p < 0.01; ### p < 0.001 vs. positive control. ^^ p < 0.01; ^^^ p < 0.001 colon cancer cells treated with extract/5-FU vs. colon epithelial cells exposed to the extract/5-FU at the corresponded concentration; one-way ANOVA test; post-test: Tukey’s.

Article Snippet: For the cell culture study, human colon adenocarcinoma cell line LS180 and human colonic epithelial cell line CCD841 CoN were purchased from the European Collection of Cell Cultures (ECACC, Centre for Applied Microbiology and Research, Salisbury, UK) and American Type Culture Collection (ATCC, Menassas, VA, USA), respectively.

Techniques: Control, Positive Control, Activity Assay, MTT Assay, Concentration Assay

Journal: International Journal of Molecular Sciences

Article Title: Phytochemical Profiling of Extracts from Rare Potentilla Species and Evaluation of Their Anticancer Potential

doi: 10.3390/ijms24054836

Figure Lengend Snippet: IC 50 values (concentration causing viability/proliferation inhibition by 50% compared to control) of aqueous acetone extracts isolated from selected Potentilla L. species. IC 50 values were calculated for human colon epithelial cell line CCD841 CoN and human colon adenocarcinoma cell line LS180 based on results of MTT as well as BrdU assays performed after 48 h of cells’ treatment with investigated compounds.

Article Snippet: For the cell culture study, human colon adenocarcinoma cell line LS180 and human colonic epithelial cell line CCD841 CoN were purchased from the European Collection of Cell Cultures (ECACC, Centre for Applied Microbiology and Research, Salisbury, UK) and American Type Culture Collection (ATCC, Menassas, VA, USA), respectively.

Techniques: Concentration Assay, Inhibition, Control, Isolation, MTT Assay, BrdU Staining

Journal: International Journal of Molecular Sciences

Article Title: Phytochemical Profiling of Extracts from Rare Potentilla Species and Evaluation of Their Anticancer Potential

doi: 10.3390/ijms24054836

Figure Lengend Snippet: Antiproliferative effect of extracts on CCD841 CoN and LS180 cell lines. The cells were exposed for 48 h to the culture medium alone (control). extract at concentrations of 25–250 µg/mL. or 25 μM 5-fluorouracil (5-FU; positive control). The antiproliferative impact of investigated compounds was measured using BrdU assay (incorporation of BrdU to newly synthesised DNA). Results are presented as mean ± SEM of at least 4 measurements. * p < 0.05; ** p < 0.01; *** p < 0.001 vs. control. # p < 0.05; ## p < 0.01; ### p < 0.001 vs. positive control. ^ p < 0.05; ^^ p < 0.01; ^^^ p < 0.001 colon cancer cells treated with extract/5-FU vs. colon epithelial cells exposed to the extract/5-FU at the corresponded concentration; one-way ANOVA test; post-test: Tukey’s.

Article Snippet: For the cell culture study, human colon adenocarcinoma cell line LS180 and human colonic epithelial cell line CCD841 CoN were purchased from the European Collection of Cell Cultures (ECACC, Centre for Applied Microbiology and Research, Salisbury, UK) and American Type Culture Collection (ATCC, Menassas, VA, USA), respectively.

Techniques: Control, Positive Control, BrdU Staining, Concentration Assay

Journal: International Journal of Molecular Sciences

Article Title: Phytochemical Profiling of Extracts from Rare Potentilla Species and Evaluation of Their Anticancer Potential

doi: 10.3390/ijms24054836

Figure Lengend Snippet: The influence of extracts on cell membrane integrity of CCD841 CoN and LS180 cell lines. The cells were exposed for 48 h to the culture medium alone (control). extract at concentrations of 25–250 µg/mL. or 25 μM 5-fluorouracil (5-FU; positive control). Extracts’ cytotoxicity (level of LDH released into the cell culture medium from damaged cell membranes) was measured using an LDH assay. Results are presented as mean ± SEM of at least 3 measurements. * p < 0.05; ** p < 0.01; *** p < 0.001 vs. control. # p < 0.05; ## p < 0.01; ### p < 0.001 vs. positive control. ^^^ p < 0.001 colon cancer cells treated with extract/5-FU vs. colon epithelial cells exposed to the extract/5-FU at the corresponded concentration; one-way ANOVA test; post-test: Tukey’s.

Article Snippet: For the cell culture study, human colon adenocarcinoma cell line LS180 and human colonic epithelial cell line CCD841 CoN were purchased from the European Collection of Cell Cultures (ECACC, Centre for Applied Microbiology and Research, Salisbury, UK) and American Type Culture Collection (ATCC, Menassas, VA, USA), respectively.

Techniques: Membrane, Control, Positive Control, Cell Culture, Lactate Dehydrogenase Assay, Concentration Assay

Journal: Cell Death & Disease

Article Title: Expansion of monocytic myeloid-derived suppressor cells ameliorated intestinal inflammatory response by radiation through SOCS3 expression

doi: 10.1038/s41419-021-04103-x

Figure Lengend Snippet: A Western blot analyses were used to determine the expression patterns of IL10-stimulated p-STAT3 and SOCS3 protein levels in CCD841 and IEC-6 cells. B , C Western-blot analysis was used to determine the expression of p-STAT3, STAT3, SOCS3, IL10, and IL6 in co-cultured IEC-6 cells with 0.5 Gy/MDSCs or 5 Gy/MDSCs directly or indirectly using a transwell system. D The expression of p-STAT3, SOCS3, IL10, and IL6 in IEC-6 cells depleted of SOCS3 using siRNA and co-cultured with splenic 0.5 Gy/MDSCs after IR exposure were determined using western blot analysis. E The expression of p-STAT3, SOCS3, IL10, and IL6 in IEC-6 cells depleted of SOCS3 using siRNA and co-cultured with recombinant IL10 after IR exposure were determined using western blot analysis. F Representative immunofluorescence images of spleen cells stained for phosphorylated STAT3 (green), IL6 (red), and both p-STAT3 and IL6 (yellow). DAPI staining (blue) was used to determine the number of nuclei. Scale bar = 100 μm. IR irradiation.

Article Snippet: The normal human colon epithelial cell line CCD841 (CRL-1790), the rat small intestinal epithelial cell line IEC-6 (CRL-1592), and mouse-derived endothelial cell line C166 (CRL-2581) were obtained from the American Type Culture Collection (Manassas, VA, USA).

Techniques: Western Blot, Expressing, Cell Culture, Recombinant, Immunofluorescence, Staining, Irradiation